ChloroquineV1, Main, Exploration, bibRecord, 001E00

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells

Identifieur interne : 001E00 ( Main/Exploration ); précédent : 001D99; suivant : 001E01

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells

Auteurs : CHUANDONG FAN [République populaire de Chine] ; WEIWEI WANG [République populaire de Chine] ; BAOXIANG ZHAO [République populaire de Chine] ; SHANGLI ZHANG [République populaire de Chine] ; JUNYING MIAO [République populaire de Chine]

Source :

Bioorganic & medicinal chemistry [ 0968-0896 ] ; 2006.

RBID : Pascal:06-0374731

Descripteurs français

Pascal (Inist)
- Quinoléine dérivé, Cytotoxicité, Mécanisme action, Mort cellulaire, Cellule tumorale, Lignée cellulaire, Amine tertiaire, Anticancéreux, Apoptose, In vitro, Poumon, Homme, Lignée A549, Chloroquine diphosphate.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Antineoplastic agent, Apoptosis, Cell death, Cell line, Cytotoxicity, Human, In vitro, Lung, Mechanism of action, Quinoline derivatives, Tertiary amine, Tumor cell.

Abstract

To investigate the effects of chloroquine diphosphate (CQ) on lung cancer cell growth, we treated A549 cells, a lung cancer cell line, with the drug at various concentrations (0.25-128 μM) for 24-72 h. The results showed that, at lower concentrations (from 0.25 to 32 μM), CQ inhibited the growth of A549 cells and, at the same time, it induced vacuolation with increased volume of acidic compartments (VAC). On the other hand, at higher concentrations (64-128 μM), CQ induced apoptosis at 24 h, while its effect of inducing vacuolation declined. The lactate dehydrogenase (LDH) assay showed that with the treatment of CQ 32-64 μM for 72 h or 128 μM for 48 h, CQ induced necrosis of A549 cells. To understand the possible mechanism by which CQ acts in A549 cells, we further incubated the cells with this drug at the concentrations of 32 or 128 μM in the presence of D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). The results showed that D609 (50 μM) could inhibit the effects of CQ 32 μM on the viability and VAC, but it could not change the effects of CQ 128 μM on the same. Our data suggested that CQ inhibited A549 lung cancer cell growth at lower concentrations by increasing the volume of lysosomes and that PC-PLC might be involved in this process. The data also indicated that, at higher concentrations, CQ induced apoptosis and necrosis, but at this time its ability to increase the volume of lysosome gradually declined, and PC-PLC might not be implicated in the process.

Affiliations:

République populaire de Chine

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Le document en format XML

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<front><div type="abstract" xml:lang="en">To investigate the effects of chloroquine diphosphate (CQ) on lung cancer cell growth, we treated A549 cells, a lung cancer cell line, with the drug at various concentrations (0.25-128 μM) for 24-72 h. The results showed that, at lower concentrations (from 0.25 to 32 μM), CQ inhibited the growth of A549 cells and, at the same time, it induced vacuolation with increased volume of acidic compartments (VAC). On the other hand, at higher concentrations (64-128 μM), CQ induced apoptosis at 24 h, while its effect of inducing vacuolation declined. The lactate dehydrogenase (LDH) assay showed that with the treatment of CQ 32-64 μM for 72 h or 128 μM for 48 h, CQ induced necrosis of A549 cells. To understand the possible mechanism by which CQ acts in A549 cells, we further incubated the cells with this drug at the concentrations of 32 or 128 μM in the presence of D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). The results showed that D609 (50 μM) could inhibit the effects of CQ 32 μM on the viability and VAC, but it could not change the effects of CQ 128 μM on the same. Our data suggested that CQ inhibited A549 lung cancer cell growth at lower concentrations by increasing the volume of lysosomes and that PC-PLC might be involved in this process. The data also indicated that, at higher concentrations, CQ induced apoptosis and necrosis, but at this time its ability to increase the volume of lysosome gradually declined, and PC-PLC might not be implicated in the process.</div>
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<name sortKey="Shangli Zhang" sort="Shangli Zhang" uniqKey="Shangli Zhang" last="Shangli Zhang">SHANGLI ZHANG</name>
<name sortKey="Weiwei Wang" sort="Weiwei Wang" uniqKey="Weiwei Wang" last="Weiwei Wang">WEIWEI WANG</name>
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Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri